Murine cell surface transferrin receptor: Studies with an anti‐receptor monoclonal antibody
- 1 September 1982
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 112 (3), 403-410
- https://doi.org/10.1002/jcp.1041120314
Abstract
A rat monoclonal antibody against the murine transferrin receptor has been identified. The receptor is a 95,000 molecular weight species that exists in the cell membrane as a disulphide-bonded dimer. Whereas 29 of 29 murine hematopoietic tumor cell lines express detectable numbers of transferrin receptors, less than 1% of adult thymocytes or spleen cells and only 5% of bone marrow cells are positive. However, fetal liver and neonatal spleen contain substantial numbers of transferrin receptor-positive cells. Induction of Friend cells in vitro with dimethyl-sulphoxide leads to an overall increase in the expression of transferrin receptors on the cell surface. The anti-transferin receptor antibody we have obtained partially blocks iron uptake from 59Fe-transferrin by a variety of murine cell lines and inhibits the growth of a murine myeloma cell line in vitro.This publication has 38 references indexed in Scilit:
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