Effect of season of collection of explants on micropropagation ofChrysanthemum morifolium
- 1 January 1988
- journal article
- research article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 30 (1), 20-24
- https://doi.org/10.1007/bf02876418
Abstract
The season of collection of shoot tips from field-grown plants ofChrysanthemum morifolium cv. Birbal Salmi was found to be crucial for their proliferation and establishment of plants in vitro. Shoot tips collected only during the period of March to April proliferated and survived. Shoot apices and segments of leaf, stem (nodal und internodal) and root, excised from aseptically established plants, were cultured on Murashige and Skoog’s medium supplemented with different concentrations and combinations of Kn, BAP, IAA and NAA. A maximum number of 9 off-shoots differentiated, without intervening callus formation, from a shoot tip in a treatment containing 1.5 mg l−1 BAP and 0.1 mg−1 NAA in 60 days. A single-node stem segment produced less shoots in the same treatment. Other kinds of expiants produced only callus. About 2-cm-long shoots, excised from cultures of proliferating shoots, were rooted 100 %, acclimatized and grown in soil. They grew normally and flowered true-to-type.Keywords
This publication has 10 references indexed in Scilit:
- Micropropagation of Rosmarinus officinalis L.Plant Cell, Tissue and Organ Culture (PCTOC), 1984
- GROWTH AND BEHAVIOR OF CELL CULTURES: EMBRYOGENESIS AND ORGANOGENESISPublished by Elsevier ,1981
- Tissue culture propagation of tropical foliage plantsIn Vitro Cellular & Developmental Biology - Plant, 1976
- PLANTLETS FROM PETAL SEGMENTS, PETAL EPIDERMIS, AND SHOOT TIPS OF THE PERICLINAL CHIMERA, CHRYSANTHEMUM MORIFOLIUM ‘INDIANAPOLIS'American Journal of Botany, 1976
- Vegetative propagation of Chrysanthemum morifolium Ram. in vitroScientia Horticulturae, 1975
- Propagation of Chrysanthemum in vitro: II. Production, Growth, and Flowering of Plantlets from Tissue Cultures1Journal of the American Society for Horticultural Science, 1974
- Rapid Multiplication of Chrysanthemum Plants by Stem-Tip Proliferation1HortScience, 1972
- Shoot Formation in Tissue Cultures of Chrysanthemum‘Bronze Pride’Physiologia Plantarum, 1968
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962
- The Culture of Excised Tissue 3Lilium speciosumThunJournal of Experimental Botany, 1957