Bromine Analysis in 5-Bromouracil-Labeled DNA by X-ray Fluorescence
- 27 December 1963
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 142 (3600), 1670-1673
- https://doi.org/10.1126/science.142.3600.1670
Abstract
The degree of replacement of thymine by 5-bromouracil in the deoxyribonucleic acid (DNA) of the thymine-deficient bacterium Escherichia coli B 15 T- has been determined by a nondispersive x-ray fluorescence method. A measure of the fraction by weight of bromine in the purified DNA is required for determining the degree of thymine replacement. This fraction is experimentally determined on 35-µg samples of DNA by x-ray spectroanalysis.Keywords
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