Antigen-Specific T Cell Factors: A Fine Analysis of Specificity

Abstract

The specificity of T cell factors produced in presence of synthetic polypeptide antigens was studied. Factors prepared with either one of the three antigens: poly(Tyr,Glu)-poly(DLAla)--poly(Lys), (T,G)-A--L, poly(Phe,Glu)-poly(DLAla)--poly(Lys), (Phe,G)-A--L, and poly(His,Glu)-poly(DLAla)--poly(Lys), (H,G)-A--L, successfully cooperated with B cells for antibody production to the homologous as well as to the other two immunogens. Furthermore, the activity of a (T,G)-A--L-specific factor was removed after passage through immunoadsorbents built of Sepharose coupled to: (T,G)-A--L, (Phe,G)-A--L and poly(Glu)-poly(DLAla)--poly(Lys), (G)-A--L, but not to poly(DLAla)--poly(LLys), A--L. No cross-reactivity was observed between (T,G)-A--L and poly(Tyr,Glu)-poly(Pro)--poly(Lys), (T,G)-Pro--L, at the level of T cell factors, as shown using the above approaches. These results lead to the conclusion that specificity of T cell factors, although not identical, is similar to that of antibodies.