The use of fluorine-containing carboxylic acids for the large-scale isolation of highly polymerized deoxyribonucleic acids

Abstract

The precipitation of the proteins of calf-thymus nucleohistone and herring-sperm nucleoprotamine with the salts of fluorine-containing carboxylic acids has been studied. Pentadecafluoro-octanoate gave a precipitate that could be sedimented almost completely by centrifuging at 2350 g for 20 min. co [omega]-H-Hexadecafluorononanoate,[omega] -H-eicosafluoro undecanoate and dodecyl sulphate gave precipitates that could not only be partly sedimented under these conditions. A method has been developed for the isolation of highly polymerized DNA on a large scale from calf thymus and herring sperm by the use of pentadecafluorooctanoate. This is more convenient and less tedious than existing methods.