The Use of Immunoadsorbent Columns for the Isolation of Antibodies Specific for Antigens Associated with Human Bronchogenic Carcinoma
Open Access
- 1 March 1974
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 29 (3), 183-188
- https://doi.org/10.1038/bjc.1974.57
Abstract
An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.Keywords
This publication has 9 references indexed in Scilit:
- Antigen Solubilized from Human Solid Tumours: Lymphocyte Stimulation and Cutaneous Delayed HypersensitivityNature New Biology, 1973
- Immunologic studies of human serous cystadenocarcinoma of ovary.Demonstration of tumor-associated antigensCancer, 1973
- Antigen Solubilized from Human Leukemia: Lymphocyte StimulationScience, 1972
- In vitro study of cellular immunity against autochthonous human cancerInternational Journal of Cancer, 1972
- Salt Extraction of Soluble HL-A AntigensScience, 1971
- SERUM-MEDIATED PROTECTION OF NEOPLASTIC CELLS FROM INHIBITION BY LYMPHOCYTES IMMUNE TO THEIR TUMOR-SPECIFIC ANTIGENSProceedings of the National Academy of Sciences, 1969
- IMMUNOCHEMICAL STUDIES ON HUMAN LUNG CANCER ANTIGENS SOLUBLE IN 50 PER CENT SATURATED AMMONIUM SULFATE1968
- DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUESThe Journal of Experimental Medicine, 1965