A Rapid, Simple Method for Nuclei Isolation from Plant Protoplasts
- 1 August 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 60 (2), 179-181
- https://doi.org/10.1104/pp.60.2.179
Abstract
A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.This publication has 4 references indexed in Scilit:
- Isolation and Properties of Nuclei from Control and Auxin-treated Soybean HypocotylPlant Physiology, 1975
- DNA replication in isolated HeLa cell nucleiBiochemical and Biophysical Research Communications, 1973
- Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota)Plant Physiology, 1972
- The Effects of Amino Acids and Ammonium on the Growth of Plant Cells in Suspension CulturePlant Physiology, 1970