Analysis of Theophylline in Serum and Whole Blood Samples by High-Pressure Liquid Chromatography
- 1 September 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 1 (5), 204-207
- https://doi.org/10.1093/jat/1.5.204
Abstract
A high-pressure liquid chromatographic procedure for theophylline, which can be applied to both ante-mortem and post-mortem samples, is described. The procedure routinely requires a 250µl sample but as little as 50µl of serum can be analyzed. Analytical results obtained using the described high-pressure liquid chromatographic procedure are compared with those obtained using a spectrophotometric method. In addition, whole-blood and sera specimens collected at the same time were analyzed and the results compared. Good correlation was observed between the high-pressure liquid chromatographic and spectrophotometric method. It was also found that the results obtained from the analysis of whole blood were comparable to the serum theophylline concentrations.This publication has 10 references indexed in Scilit:
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