Cloning and characterization of the xyl genes from Escherichia coli
- 1 May 1984
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 194 (3), 410-415
- https://doi.org/10.1007/bf00425552
Abstract
Summary Specific xylose utilization mutants of Escherichia coli were isolated that had altered xylose isomerase (xylA), xylulokinase (xylB), and regulatory (xylR) or transport (xylT) activities. We screened the Clarke and Carbon E. coli gene bank and one clone, pLC10–15, was found to complement the xyl mutants we had characterized. Subcloning and DNA restriction mapping allowed us to locate the xylA and xylB genes on a 1.6 kbp BglII fragment and a 2.6 kbp HindIII-SalI fragment, respectively. The identification and mapping of xyl gene promoters suggest that the xylA and xylB genes are organized as an operon having a single xylose inducible promoter preceding the xylA gene.Keywords
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