TRANSDUCTION BY BACTERIOPHAGE P1: ABNORMAL PHAGE FUNCTION OF THE TRANSDUCING PARTICLES

Abstract
To investigate transduction by phage P1 a study was made of the segregation of non-phage -carrier cells of Shigella dysenteriae Sh and E. coli K-12 after infection with phages Pl or Pike. The segregation patterns in clones from unselected infected cells were compared to those from transduced cells. Practically all unselected infected cells that did not lyse give pure lysogenic clones. The results from colonies selected for transduction are different: in most instances the transduced clones are phage-sensitive, in a few transductions the clones are phage-immune, defective-lysogenic. Three explanations are proposed.