Domain II of calmodulin is involved in activation of calcineurin
- 26 September 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 238 (1), 82-86
- https://doi.org/10.1016/0014-5793(88)80230-8
Abstract
A family of mutant proteins related to calmodulin (CaM) has been produced using cDNA constructs in bacterial expression vectors. The new proteins contain amino acid substitutions in Ca2+-binding domains I, II, both I and II, or both II and IV. The calmodulin-like proteins have been characterized with respect to mobility on SDS-polyacrylamide gels, Ca2+-dependent enhancement of tyrosine fluorescence, and abilities to activate the CaM-dependent phosphatase calcineurin. These studies suggest that an intact Ca2+-binding domain II is minimally required for full activation of calcineurin.Keywords
This publication has 19 references indexed in Scilit:
- Fluorescence anisotropy decay demonstrates calcium-dependent shape changes in photo-cross-linked calmodulinBiochemistry, 1988
- Stimulation of the erythrocyte Ca2+‐ATPase and of bovine brain cyclic nucleotide phosphodiesterases by chemically modified calmodulinEuropean Journal of Biochemistry, 1987
- Affinity selection of chemically modified proteins: role of lysyl residues in the binding of calmodulin to calcineurinBiochemistry, 1987
- [34]Specific chemical modification as a probe of calmodulin functionMethods in Enzymology, 1987
- Association of calmodulin and smooth muscle myosin light chain kinase: Application of a lable selection technique with trace acetylated calmodulinProteins-Structure Function and Bioinformatics, 1987
- Selective effects of CAPP1-calmodulin on its target proteinsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- CAPP-calmodulin: A potent competitive inhibitor of calmodulin actionsFEBS Letters, 1984
- Tissue-specific expression of a chicken calmodulin pseudogene lacking intervening sequences.Proceedings of the National Academy of Sciences, 1983
- Calculation of the concentrations of free cations and cation-ligand complexes in solutions containing multiple divalent cations and ligandsBiophysical Journal, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970