Swi5 Controls a Novel Wave of Cyclin Synthesis in Late Mitosis
- 1 April 1998
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 9 (4), 945-956
- https://doi.org/10.1091/mbc.9.4.945
Abstract
We have shown previously that the Swi5 transcription factor regulates the expression of the SIC1 Cdk inhibitor in late mitosis. This suggests that Swi5 might control other genes with roles in ending mitosis. We identified a gene with a Swi5-binding site in the promoter that encoded a protein with high homology to Pcl2, a cyclin-like protein that associates with the Cdk Pho85. This gene,PCL9, is indeed regulated by Swi5 in late M phase, the only cyclin known to be expressed at this point in the cell cycle. The Pcl9 protein is associated with a Pho85-dependent protein kinase activity, and the protein is unstable with peak levels occurring in late M phase. PCL2 is already known to be expressed in late G1 and we find that, in addition, it is also regulated by Swi5 in telophase. The expression of PCL2 andPCL9 at this stage of the cell cycle implies a role for the Pho85 Cdk at the end of mitosis. Consistent with this a synthetic interaction was observed between pho85Δ and strains deleted for SIC1, SWI5, and SPO12. These and other studies support the notion that the M/G1 switch is a major cell cycle transition.Keywords
This publication has 41 references indexed in Scilit:
- Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.Genes & Development, 1996
- Identification of an Asymmetrically Localized Determinant, Ash1p, Required for Lineage-Specific Transcription of the Yeast HO GeneCell, 1996
- Asymmetric Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother CellsCell, 1996
- An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeastNature, 1996
- CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transitionCell, 1995
- The PCL2 (ORFD)-PHO85 Cyclin-Dependent Kinase Complex: a Cell Cycle Regulator in YeastScience, 1994
- Cell Cycle Control by a Complex of the Cyclin HCS26 (PCL1) and the Kinase PHO85Science, 1994
- P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae.Genes & Development, 1994
- Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase.Genes & Development, 1992
- The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle controlGene, 1991