Studies on the synergistic effect of amphotericin B and 5-fluorocytosine on the growth rate of single hyphae of Aspergillus fumigatus by a biocell-tracer system

Abstract

The biocell-tracer system is a microscopical system to measure the growth rate of a single fungal hypha. The synergistic effect of amphotericin B (AMPH) and 5-fluorocytosine (5-FC) on the growth of hyphae of Aspergillus fumigatus was studied by using this system. Although neither 2 μg/ml of AMPH nor 250 μg/ml of 5-FC alone showed any effect on the hyphal growth, their combination at these concentrations showed a distinct inhibitory activity. The biocell-tracer system is useful for antifungal activity testing in filamentous fungi.