Selective degradation of thymidine and thymine deoxynucleotides
- 1 January 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 98 (1), 70-77
- https://doi.org/10.1042/bj0980070
Abstract
Osmium tetroxide in dilute ammonia oxidizes various pyrimidine nucleosides at different rates. Thymidine is oxidized about 45 times as fast as deoxycytidine. The phosphate groups may be eliminated from oxidized thymine nucleotides by successive treatments with alkali and then with diphenylamine in aqueous formic acid. The reactions can be applied to the selective degradation of thymidine in oligodeoxnyncleotides.This publication has 20 references indexed in Scilit:
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