Biological responses of Bacillus stratosphericus to Floating Electrode-Dielectric Barrier Discharge Plasma Treatment
- 12 November 2010
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 109 (6), 2039-2048
- https://doi.org/10.1111/j.1365-2672.2010.04834.x
Abstract
Dielectric barrier discharge (DBD) plasma is used for sterilization of contaminated inanimate surfaces but seldomly optimized and depends upon the type of organisms and the plasma treatment duration, (net energy deposited) this efficacy varies. The proposed study was designed to see biological responses of one of the robust organism, Bacillus stratosphericus. DBD plasma was applied over various durations to B. stratosphericus either surface-dried or suspension in de-ionized water, and viability, culturability, and viable but nonculturability (VBNC) were assayed using standard techniques. Depending upon the exposure of B. stratosphericus to DBD plasma resulted in three viability states, viable and culturable at low plasma doses and VBNC or disintegrated bacteria at higher plasma doses. Although organism's respiration levels at relatively low levels, immediately after plasma treatment, over the course of 24-h respiratory activity was increased c. eight times (and found still nonculturable during colony assays). The loss of culturability is hypothesized to be induced as one of the responses to oxidative stress and it remains to be unclear if the response is temporary or indefinite. Appropriate plasma powers should be used to avoid VBNC-like status. 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay is a good alternative method to detect VBNC state. Bacillus stratosphericus has the potential to turn into VBNC upon plasma application, and XTT assay can be an alternative method to detect VBNC state.Keywords
This publication has 31 references indexed in Scilit:
- Physical and biological mechanisms of direct plasma interaction with living tissueNew Journal of Physics, 2009
- Decontamination of Surfaces From Extremophile Organisms Using Nonthermal Atmospheric-Pressure PlasmasIEEE Transactions on Plasma Science, 2009
- Heating Effect of Dielectric Barrier Discharges for Direct Medical TreatmentIEEE Transactions on Plasma Science, 2008
- Plasma Biology and Plasma MedicinePublished by Cambridge University Press (CUP) ,2008
- Critical Evaluation of Two Primers Commonly Used for Amplification of Bacterial 16S rRNA GenesApplied and Environmental Microbiology, 2008
- Comparison of Direct and Indirect Effects of Non‐Thermal Atmospheric‐Pressure Plasma on BacteriaPlasma Processes and Polymers, 2007
- Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation?The ISME Journal, 2007
- Blood Coagulation and Living Tissue Sterilization by Floating-Electrode Dielectric Barrier Discharge in AirPlasma Chemistry and Plasma Processing, 2006
- Visible light damage to Escherichia coli in seawater: oxidative stress hypothesisJournal of Applied Bacteriology, 1994
- BIOCHEMICAL COMPOSITION AND METABOLIC ACTIVITY OF SCRIPPSIELLA TROCHOIDEA (DINOPHYCEAE) RESTING CYSTS1Journal of Phycology, 1990