The beta-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid.
- 1 August 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (16), 6338-6342
- https://doi.org/10.1073/pnas.86.16.6338
Abstract
In this study, we use antisera to synthetic .beta.-amyloid protein precursor (.beta.APP) peptides to identify, in human brain and cerebrospinal fluid (CSF), soluble .apprxeq. 125- and .apprxeq. 105-kDa derivatives of the .beta.APP that lack the carboxyl terminus of the full-length, membrane-associated forms. We show that the soluble .apprxeq. 125-kDa .beta.APP derivative contains the Kunitz protease inhibitor domain, whereas the .apprxeq. 105-kDa form does not, and we confirm that these two proteins are soluble .beta.APP derivatives by purifying each from human CSF and directly sequencing its amino terminus.This publication has 29 references indexed in Scilit:
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