GABA transport in the rat thyroid
- 1 August 1981
- journal article
- research article
- Published by Springer Nature in Naunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie
- Vol. 317 (1), 61-66
- https://doi.org/10.1007/bf00506258
Abstract
The uptake of γ-aminobutyric acid (GABA) into rat thyroid slices was studied. Uptake of 14C-GABA was concentration-dependent: one unsaturable (diffusion) and two saturable components obeying Michaelis-Menten kinetics contributed to transport. The kinetic constants of saturable GABA transport systems were: K m1 =1.5 μM, V 1=4.0 nmol×(g wet weight)−1×(20 min)−1 (high-affinity uptake); K m2 =800 μM, V 2=260 nmol×(g wet weight)−1×(20 min)−1 (low-affinity uptake). Uptake mediated by each of the carrier systems was concentrative, entirely Na+-dependent, and required activation energies characteristic for active transport. High-affinity transport was structurally specific for GABA. The substrate specificity of low-affinity uptake resembled that of β-amino acid transport systems.Keywords
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