High affinity interaction of mammalian DNA topoisomerase I with short single‐ and double‐stranded oligonucleotides
- 10 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (1), 97-100
- https://doi.org/10.1016/0014-5793(95)00609-d
Abstract
The interaction of DNA topoisomerase I (topo I) with a set of single- and double-stranded oligonucleotides containing 5–27 mononucleotides was investigated. All single- and double-stranded oligonucleotides were found to inhibit competitively the supercoiled DNA relaxation reaction catalyzed by topo I. The enzyme affinity for specific sequence pentanucleotides of the scissile (GACTT, Ki = 2 μM) and non-cleaved chain (AAGTC, Ki = 110 μM) is about 2–4 orders of magnitude higher than that for non-specific oligonucleotides. This specific sequence affinity increases in several cases: lengthening of single-stranded oligonucleotides, formation of stable duplexes between complementary oligonucleotides and preincubation of the enzyme with ligands before addition of supercoiled DNA. We assume that oligonucleotides having a high affinity to the enzyme can offer a unique opportunity for rational design of topoisomerase-targeting drugsKeywords
This publication has 19 references indexed in Scilit:
- Induction of mammalian DNA topoisomerase I mediated DNA cleavage by antitumor indolocarbazole derivativesBiochemistry, 1992
- New technique for uncoupling the cleavage and religation reactions of Eukaryotic Topoisomerase I.Journal of Molecular Biology, 1991
- Analysis of melting transitions of the DNA hairpins formed from the oligomer sequences d[GGATAC(X)4GTATCC] (X = A, T, G, C)Biopolymers, 1990
- The role of DNA topoisomerases in recombination and genome stability: A double-edged sword?Cell, 1990
- DNA TOPOISOMERASE POISONS AS ANTITUMOR DRUGSAnnual Review of Biochemistry, 1989
- Biochemical basis for the interactions of type I and type II topoisomerases with DNAPharmacology & Therapeutics, 1989
- DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombeCell, 1987
- DNA TOPOISOMERASESAnnual Review of Biochemistry, 1985
- Breakage of single-stranded DNA by eukaryotic type 1 topoisomerase occurs only at regions with the potential for base-pairingJournal of Molecular Biology, 1984
- DNA TopoisomerasesAnnual Review of Biochemistry, 1981