A Controlled Environmental Culture System for the Production of Erythropoietin

Abstract

A controlled environmental culture system in which consistent growth of mouse fetal liver cells typical of the tissue of origin and capable of producing erythropoietin in culture is described. This system attempts to duplicate the major components of the natural habitat of the mammalian cell allowing for meaningful studies to be conducted on the regulation and control of erythropoietin biosynthesis. The study of direct effects of changes in pO2 [O2 tension], pCO2 [CO2 tension], pH and various cell nutrients on cell producing erythropoietin is now possible.