Präparative Auftrennung des tryptischen Hydrolysats eines Proteins mit Hilfe der Hochdruck-Flüssigkeitschromatographie. Die Primärstruktur einer monoklonalen L-Kette vom K-Typ, Subgruppe I (Bence-Jones-Protein Wes)

Abstract

The tryptic hydrolysate of [human] Bence-Jones protein Wes (MW = 23,000) was separated by high-pressure liquid chromatography in a volatile buffer system on a reversed-phase column. From the resulting 22 peptides 18 yielded integer numbers after amino acid analysis. They could be used directly for a modified Edman degradation. Two peptides were not separated by this procedure, and 2 were missing. Under different conditions these 4 peptides could also be prepared in pure form. Three mg was sufficient to elucidate the primary structure of the variable part of this protein completely. The arrangement of the tryptic peptides was deduced by homology to other .kappa.-chains. Protein Wes contains 214 residues and belongs to subgroup I of the .kappa.-chains. The valine residue in position 191 indicates that it belongs to allotype Inv b+.