The stereochemical course of hydrolysis catalysed by snake venom 5′-nucleotide phosphodiesterase
- 1 November 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 199 (2), 447-451
- https://doi.org/10.1042/bj1990447
Abstract
Adenosine 5′-(S)-[16O,17O,18O]phosphate was pyrophosphorylated by the combined action of adenylate kinase and pyruvate kinase. The isotopomers of adenosine 5′-[alpha-16O,17O,18O]triphosphate were hydrolysed by venom 5′-nucleotide phosphodiesterase (Crotalus adamanteus) in H2(17)O. Analysis by 31P nuclear magnetic resonance spectroscopy of the resulting adenosine 5′-[16O,17O,18O]phosphate, after cyclization and esterification, showed that the hydrolysis occurs with retention of configuration at phosphorus. The most likely explanation of this observation is that the enzymic hydrolysis involves a double displacement at phosphorus with a covalent nucleotidyl--enzyme intermediate on the reaction pathway.This publication has 13 references indexed in Scilit:
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