Abstract
Ethanol-grown cells of A. peroxydans oxidize ethanol, pyruvate, succinate, malate, fumarate, and oxalacetate, but not acetate, to CO2 and water. Crude cell-free preparations oxidize ethanol only to acetate, and the tricarboxylic acid cycle intermediates weakly, if at all, past oxalacetate. In extracts, ethanol dehydrogenase is DPN-linked and cyanide-insensitive, whereas DPNH oxidation is inhibited by cyanide.