Simple and rapid method for isolating large plasmid DNA from lactic streptococci
- 1 September 1983
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 46 (3), 549-552
- https://doi.org/10.1128/aem.46.3.549-552.1983
Abstract
A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed. ImagesThis publication has 18 references indexed in Scilit:
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