Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides
Open Access
- 11 May 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (9), 2575-2584
- https://doi.org/10.1093/nar/11.9.2575
Abstract
The paper describes an improved method for the synthesis of oligodeoxyribo-nucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3′-morpholino-meth-oxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.Keywords
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