Depolarizing stimuli regulate nerve growth factor gene expression in cultured hippocampal neurons.
- 15 July 1991
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (14), 6289-6292
- https://doi.org/10.1073/pnas.88.14.6289
Abstract
Although trophic factors and neuronal activity have been implicated in regulating functional synaptic circuits, the relationship of trophic interaction to impulse activity in synaptogenesis remains unclear. Using cultured hippocampus as a model system, we provide direct evidence that depolarization and impulse activity specifically increase nerve growth factor gene expression in neurons. Depolarizing stimuli, such as a high K+ concentration or the Na+ channel agonist veratridine, elicited a 3-fold increase of nerve growth factor mRNA levels in both explant and dissociated cultures. Blockade of depolarization by tetrodotoxin prevented the increase of neuronal nerve growth factor mRNA. Further, nerve growth factor gene expression was stimulated by picrotoxin, a gamma-aminobutyric acid antagonist frequently used to enhance hippocampal neuronal activity. Impulse regulation of trophic gene function may be relevant to developmental synaptogenesis and synaptic strengthening in learning and memory.Keywords
This publication has 26 references indexed in Scilit:
- Expression of NGF and NGF receptor mRNAs in the developing brain: Evidence for local delivery and action of NGFExperimental Neurology, 1989
- Synaptic Connections in Vitro: Modulation of Number and Efficacy by Electrical ActivityScience, 1989
- Strengthening the synapsesNature, 1989
- What, if anything, is a neurotrophic factor?Trends in Neurosciences, 1988
- Long-Term Synaptic PotentiationScience, 1988
- Prenatal Tetrodotoxin Infusion Blocks Segregation of Retinogeniculate AfferentsScience, 1988
- Expression of the β-Nerve Growth Factor Gene in Hippocampal NeuronsScience, 1988
- Effects of high K+ concentrations on the growth and development of ciliary ganglion neurons in cell cultureDevelopmental Biology, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Plasticity of ocular dominance columns in monkey striate cortexPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1977