Osteogenic periosteum esterase activity: a comparative morphological and cytochemical study of bone cells in situ on rat proximal tibiae and in smears.
Open Access
- 1 January 1984
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 32 (1), 55-62
- https://doi.org/10.1177/32.1.6197438
Abstract
Mononucleate phagocytes are presently thought to participate, along with osteoclasts, in bone resorption. Similar cells may also be precursors to osteoclasts. Esterase is a hydrolytic enzyme commonly found in most phagocytes and cells such as these have been shown to degrade bone matrix in vitro. Therefore, periosteal cells of remodeling proximal tibiae in rats were examined for esterase activity. Stain reactions were compared both in situ and in smears. Percentages of different cell types were obtained by counting cells in Wright-Giemsa-stained smears. Osteoclasts and mononucleate phagocyte-like cells made up approximately 20% of the isolated cells. Cells resembling preosteoblasts, mononucleate phagocytes, and osteoblasts stained for esterase activity with light to moderate intensity. Osteoclasts and mononucleate osteoclast-like cells were strongly esterase positive. The esterase was characterized as being type B by using nonorganophosphorus inhibitors of the various esterase types. The addition of esterase to the list of enzyme activities of cells in close proximity to osteoclasts lends further support to the hypothesis that mononucleate cells also participate in bone resorption.This publication has 26 references indexed in Scilit:
- Disappearing bone disease. A clinical and histological studyJournal of Bone and Joint Surgery, 1977
- THYMIDINE-3H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RATThe Journal of cell biology, 1967
- The occurrence of specific cytoplasmic granules in the osteoclastJournal of Ultrastructure Research, 1967
- THE ORIGIN AND TURNOVER OF MONONUCLEAR CELLS IN PERITONEAL EXUDATES IN RATSThe Journal of Experimental Medicine, 1966
- Distribution of esterases in rat liverArchives of Biochemistry and Biophysics, 1964
- CELL PROLIFERATION AND SPECIALIZATION DURING ENDOCHONDRAL OSTEOGENESIS IN YOUNG RATSThe Journal of cell biology, 1962
- CITRIC ACID CYCLE IN OSTEOBLASTS AND OSTEOCLASTS - A HISTOCHEMICAL STUDY OF NORMAL AND PARATHORMONE-TREATED RATS1961
- CELL DIVISION IN ENDOCHONDRAL OSSIFICATIONThe Journal of Bone and Joint Surgery. British volume, 1960
- The histochemistry of giant-cell tumours (osteoclastoma and giant-cell epulis)The Journal of Pathology and Bacteriology, 1958
- THE HISTOCHEMISTRY OF INDOXYLESTERASE OF RAT KIDNEY WITH SPECIAL REFERENCE TO ITS CATHEPSIN-LIKE ACTIVITY1958