Detection of rpoB Mutations in Mycobacterium tuberculosis by Biprobe Analysis
Open Access
- 1 September 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (9), 3350-3352
- https://doi.org/10.1128/jcm.39.9.3350-3352.2001
Abstract
A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.Keywords
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