Purification of Poly-β-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide

Abstract

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-β-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.