Light-harvesting chlorophyll a / b -protein: Three-dimensional structure of a reconstituted membrane lattice in negative stain
- 1 January 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (2), 386-390
- https://doi.org/10.1073/pnas.82.2.386
Abstract
The 3-dimensional structure of a negatively stained hexagonal membrane lattice [from Pisum sativum] containing the light-harvesting chlorophyll a/b-protein complex and phospholipids was determined to 30-.ANG. resolution by image reconstruction from electron micrographs. This lattice has p321 symmetry, a lattice constant of 125 .ANG. and a thickness of 75 .ANG.. The monomer is an elongated molecule .apprx. 65 .ANG. long in the dimension perpendicular to the plane of the membrane. It spans the hydrophobic domain of the membrane in an asymmetric fashion, projecting .apprxeq. 20 .ANG. from one surface and less from the other. On the basis of this image and available biochemical data, the structure of the complex in the native thylakoid membrane is proposed.This publication has 32 references indexed in Scilit:
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