Polyamines and protein synthesis: Studies in various polyamine-requiring mutants ofEscherichia coli
- 1 July 1977
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 16 (2-3), 71-77
- https://doi.org/10.1007/bf01732046
Abstract
DifferentEscherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. Thein vitro protein synthetic activity and the polyribosomal content were reduced in extracts from putrescine-starved cells of the double mutants MA 255 and MA 261, but not in the arginine-conditional auxotroph DK 6. Putrescine addition to the cultures of all these strains previously starved for polyamines, provoked a shift towards monomers in the equilibrium involving ribosomal particles. Concomitant changes in the intracellular levels of polyamines were observed: putrescine and spermidine increased markedly, and cadaverine disappeared.Keywords
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