Influence of Cortisone on Free Hydroxyproline in the Developing Chick Embryo

Abstract

Summary A chromatographic examination was made of the free amino acids of tissues of normal and cortisone-injected chick embryos. The content of free hydroxyproline was increased markedly in the tissues of the cortisone-treated embryos. The results were confirmed by a colorimetric procedure. With the exception of glycine, in which increases were noted in some instances, the other amino acids showed no consistent changes as a result of the injection of cortisone. The injection of Δ¹-17 α-hydroxy progesterone produced slight decreases in free hydroxyproline content.