Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.
- 1 March 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (3), 1343-1347
- https://doi.org/10.1073/pnas.76.3.1343
Abstract
S. typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to 8-azaguanine resistance was measured in the treated cultures and increased linearly with AFB1 concentration. DNA purified from mutagenized cells was analyzed for AFB1 adduct formation by high-pressure liquid chromatography after adduct liberation. AFB1 exposures at 0.16 and 0.32 .mu.M for 35 min produced 15 and 22 AFB1-DNA adducts/genome, respectively, and induced 8-azaguanine-resistant fractions of 4.9 .times. 10-4 and 9.6 .times. 10-4. About 70% of the AFB1 bound to DNA was chromatographically identical to 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 at the 2 AFB1 levels used.This publication has 22 references indexed in Scilit:
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