Absence of Lipolytic Activity from Purified Human Growth Hormone in Cultured 3T3-L1 Adipocytes
- 1 January 1983
- journal article
- research article
- Published by S. Karger AG in Hormone Research
- Vol. 17 (4), 197-201
- https://doi.org/10.1159/000179698
Abstract
Although standard preparations of growth hormone (GH) activated hormone-sensitive lipase in differentiated [mouse fibroblast] 3T3-L1 adipocytes, more highly purified preparations of human and bovine hormones were not lipolytic in this system. The standard preparations were contaminated with a lipolytic substance which was removed during purification of the GH. The purified GH likewise did not stimulate lipolysis in adipose tissue from fasted rats. Dexamethasone had no potentiating activity in either assay.Keywords
This publication has 4 references indexed in Scilit:
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- Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells.Journal of Biological Chemistry, 1978