Initial characterization of monoclonal antibodies against human monocytes.

Abstract

Three monoclonal antibodies against human monocytes were produced by somatic cell fusion. Extensive specificity analysis suggests that these antibodies react with most, if not all, human peripheral blood monocytes and not with highly purified T or B cells. Initial chemical characterization of the monocyte antigen recognized by 2 of these antibodies is presented. The molecule is a single polypeptide chain with an apparent MW of 200,000. These reagents should prove useful in the clinical definition of monocyte differentiation disorders, in studies of monocyte function, and in the elucidation of the genetics and structure of monocyte cell surface antigens.