Two forms of the rat D2 dopamine receptor as revealed by the polymerase chain reaction
- 9 April 1990
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 263 (1), 18-22
- https://doi.org/10.1016/0014-5793(90)80695-f
Abstract
We have used the polymerase chain reaction technique (PCR) to clone the cDNA of the D2 dopamine receptor from rat striatal mRNA. Two major PCR products were produced; one product was identical to a previously published rat cDNA, while the other, more abundant product differed only by an 87‐nucleotide insert located in the region of the putative third cytoplasmic loop of the D2 receptor. A PCR approach for determining message abundance was used to determine the relative message abundance of the two forms of the D2 receptor in a variety of tissues. Possible implications of the two forms of the D2 receptor for dopamine‐mediated signal transduction are discussed.Keywords
This publication has 16 references indexed in Scilit:
- Identification of a small intracellular region of the muscarinic m3 receptor as a determinant of selective coupling to PI turnoverFEBS Letters, 1989
- All ras proteins are polyisoprenylated but only some are palmitoylatedCell, 1989
- Genetic Regulation of β-Adrenergic ReceptorsAnnual Review of Physiology, 1989
- Location of a region of the muscarinic acetylcholine receptor involved in selective effector couplingFEBS Letters, 1988
- Cloning and expression of a rat D2 dopamine receptor cDNANature, 1988
- Characterization of the MMQ Cell, A Prolactin-Secreting Clonal Cell Line That Is Responsive to Dopamine*Endocrinology, 1988
- Chimeric α 2 -,β 2 -Adrenergic Receptors: Delineation of Domains Involved in Effector Coupling and Ligand Binding SpecificityScience, 1988
- Structure-Function Analysis of the -Adrenergic ReceptorCold Spring Harbor Symposia on Quantitative Biology, 1988
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology, 1987
- Multiple receptors for dopamineNature, 1979