Interleukin‐33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin‐4 in naïve mice
Open Access
- 15 June 2012
- Vol. 67 (9), 1118-1126
- https://doi.org/10.1111/j.1398-9995.2012.02859.x
Abstract
Background The regulation and function of IgE in healthy individuals and in antigen‐naïve animals is not well understood. IL‐33 administration increases serum IgE in mice with unknown mechanism. We tested the hypothesis that IL‐33 provides an antigen‐independent stimulus for IgE production and mast cell degranulation. Methods IL‐33 was administered to naïve wild‐type (WT), nude and ST2−/−, IL‐4−/−, IL4Rα−/− and T‐or B‐cell‐specific IL‐4Rα−/− mice. IgEand cytokines were quantified by ELISA. T‐ and B‐lymphocyte numbers and CD40L expression were determined by flow cytometry. Anaphylaxis was measured by temperature, mast cell degranulation and histamine release. Results IL‐33 enhanced IgE production in naïve WT, T‐IL‐4Rα−/− but not in ST2−/−, IL‐4−/−, IL‐4Rα−/− or B‐cell‐specific IL‐4Rα−/− mice, demonstrating IL‐33 specificity and IL‐4 dependency. Moreover, IL‐4 was required for IL‐33‐induced B‐cell proliferation and T‐cell CD40L expression, which promotes IgE production. IL‐33‐induced IL‐4 production was mainly from innate cells including mast cells and eosinophils. IL‐33 increased mast cell surface IgE and triggered degranulation and systemic anaphylaxis in allergen‐naïve WT but not in IL‐4Rα−/− mice. Conclusion IL‐33 amplifies IgE synthesis and triggers anaphylaxis in naïve mice via IL‐4, independent of allergen. IL‐33 may play an important role in nonatopic allergy and idiopathic anaphylaxis.Keywords
This publication has 35 references indexed in Scilit:
- Immune Sensitization in the Skin Is Enhanced by Antigen-Independent Effects of IgEImmunity, 2004
- T1/ST2—an IL-1 receptor-like modulator of immune responsesCytokine & Growth Factor Reviews, 2004
- A current review of idiopathic anaphylaxisCurrent Opinion in Allergy and Clinical Immunology, 2003
- Regulation of Mast Cell Survival by IgEImmunity, 2001
- Monomeric IgE Stimulates Signaling Pathways in Mast Cells that Lead to Cytokine Production and Cell SurvivalImmunity, 2001
- How much asthma is really attributable to atopy?Thorax, 1999
- Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T CellsThe Journal of Experimental Medicine, 1998
- Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cellsImmunology Today, 1994
- Lymphokine Control of In Vivo Immunoglobulin Isotype SelectionAnnual Review of Immunology, 1990
- Induction of human IgE synthesis requires interleukin 4 and T/B cell interactions involving the T cell receptor/CD3 complex and MHC class II antigens.The Journal of Experimental Medicine, 1989