The purity of thyroglobulin isolated from normal and carcinomatous thyroid tissue on one patient by fractional salting-out with ammonium sulphate

Abstract

Thyroglobulins prepared from normal and carcinomatous thyroid tissue of one patient migrated as single components during electrophoresis in 0.05 [image] veronal buffer (pH 8.6, 10.05) at concentrations of 1 and 0.4% (w7v). They differed significantly in mobility. Analysis by the gel-diffusion method of Ouchterlony revealed the presence of 3, possibly 4, antigenic components in each thyroglobulin, but no qualitative difference between the 2 thyroglobulins.