Studies on Egg Drop Syndrome (EDS) and Chick Embryo Lethal Orphan (CELO) Avian Adenovirus DNAs by Restriction Endonucleases

Abstract

Summary Restriction endonucleases EcoRI, BamHI, HindIII, BglI, BglII, HpaI and PstI recognized 48 cleavage sites in EDS adenovirus strain B8/78 DNA, whereas CELO virus DNA was cut into 61 fragments by the same enzymes. No similarity could be detected in the restriction patterns obtained from the two avian adenoviruses. The calculated mol. wt. of B8/78 DNA was 22.6 × 106 [about 34.2 kilobases (kb)] and that of CELO virus DNA was 28.9 × 106 (about 43.7 kb). The fragments generated from B8/78 DNA by EcoRI and BamHI were physically mapped.