Regulation of expression of liver‐specific enzymes: I. Detection in mammalian tissues and cultured cells
- 1 October 1981
- journal article
- Published by Wiley in Annals of Human Genetics
- Vol. 45 (4), 341-356
- https://doi.org/10.1111/j.1469-1809.1981.tb00348.x
Abstract
Nineteen enzymes showing highest activity in liver were examined in human and rodent tissues and cultured cells using starch-gel electrophoresis. The rat hepatoma line Faza 967 strongly expressed 13 of these enzymes. A series of somatic cell hybrids, constructed between Faza and cells of non-hepatic origin derived from man or from Chinese hamster, were examined for expression of these enzymes. Some of the human/rat hybrids continued to produce rat liver-specific enzymes, and the human forms of the enzymes glutamate-pyruvate transaminase, alpha-glycerophosphate dehydrogenase, alcohol dehydrogenase and pyruvate kinase L were reexpressed in a few cases.Keywords
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