300-MHz Nuclear Magnetic Resonance Study of Oxytocin in Aqueous Solution: Conformational Implications
- 1 December 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (12), 3806-3809
- https://doi.org/10.1073/pnas.70.12.3806
Abstract
The 300-MHz nuclear magnetic resonance spectrum of the peptide hormone, oxytocin, in water, was obtained. Extensive nuclear magnetic resonance spindecoupling experiments, including those involving irradiation of the alpha-proton resonance under the water peak, studies using partially deuterated hormone derivatives, and nuclear magnetic resonance studies of some precursor peptides to oxytocin, permitted us to assign most of the proton resonance of the hormone unambiguously. The JNalpha values and temperature dependence of the chemical shift of the peptide amide and carboxamide protons of oxytocin were also obtained. These studies indicate that, in aqueous solution, oxytocin is a flexible molecule possessing several different conformations. Corresponding studies of [4-leucine]oxytocin were also made, with similar results and conclusions.Keywords
This publication has 19 references indexed in Scilit:
- Conformational Studies of Oxytocin and Lysine Vasopressin in Aqueous Solution using High Resolution NMR SpectroscopyNature New Biology, 1971
- Proposed Conformation of Oxytocin in SolutionProceedings of the National Academy of Sciences, 1971
- NMR Studies on the Conformation of Derivatives of the Side Chain of Oxytocin: Examples of cis-trans IsomerismProceedings of the National Academy of Sciences, 1971
- Secondary Structure of the Cyclic Moiety of the Peptide Hormone Oxytocin and Its Deamino AnalogProceedings of the National Academy of Sciences, 1970
- The Synthesis and Some of the Pharmacological Properties of [4-L-Isoleucine]-oxytocin and [4-L-Leucine]-oxytocinJournal of Biological Chemistry, 1969
- 4-Leucine-Oxytocin: Natriuretic, Diuretic, and Antivasopressin PolypeptideScience, 1968
- Conformational studies on neurohypophyseal hormones: the disulfide bridge of oxytocin.Proceedings of the National Academy of Sciences, 1968
- Circular Dichroism of Oxytocin and Several Oxytocin AnaloguesJournal of Biological Chemistry, 1968
- Nuclear Magnetic Resonance Study of the Protolysis Kinetics of the Peptide Hydrogens of Triglycine1aJournal of the American Chemical Society, 1966
- Simultaneous Synthesis of 1-Hemi-D-cystine-oxytocin and Oxytocin and Separation of the Diastereoisomers by Partition Chromatography on Sephadex and by Countercurrent Distribution1Journal of the American Chemical Society, 1966