Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin

Abstract

A cDNA library from membrane-bound poly(A)⁺ mRNA of developing potato tubers was constructed and two classes of essentially full-length cDNA clones for a precursor to the major tuber glycoprotein, patatin were isolated. Sequence analysis shows that the two classes are approximately 98% homologous and correspond to the two major species of patatin identified previously by NH₂-terminal amino acid sequence analysis. Sequence analysis also predicts that patatin 1s synthesized with a 23 amino acid signal sequence. Northern blot analysis shows that patatin mRNAs are 155O±5O nucleotides 1n length and are normally not present in polyribosomal or total RNA from stems or leaves.