Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin.
Open Access
- 1 June 1987
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 6 (6), 1677-1683
- https://doi.org/10.1002/j.1460-2075.1987.tb02417.x
Abstract
The gene for murine Ia‐associated invariant (Ii) chains (Ii31 and Ii41) was characterized by sequence analysis. The gene extends over approximately 9 kb and is organized in nine exons. Exon 1 encodes the 5′ untranslated region and the cytoplasmic segment, exon 2 the membrane spanning segment and adjacent amino acids and exons 3‐8 the extracytoplasmic portion of Ii31. Putative promoter sequences were found upstream of the start of the coding sequence. Between exons 6 and 7 an additional, alternatively spliced exon 6b has been identified. This exon is spliced into the mRNA coding for the Ii‐related Ii41 protein. Exon 6b encodes a cysteine‐rich domain of 64 amino acids. It shows a remarkably high homology to the repetitive elements in thyroglobulin, a precursor for thyroid hormone. Based on this homology, it is suggested that this domain (TgR) in Tg and in Ii41 may play a role either in hormone formation or as a carrier in the transport of molecules (thyroid hormone or processed antigen respectively) between intracellular compartments.This publication has 66 references indexed in Scilit:
- Structure, expression and regulation of the thyroglobulin geneMolecular and Cellular Endocrinology, 1985
- Analysis of cosmids using linearization by phage lambda terminaseGene, 1985
- Mouse prepro-epidermal growth factor synthesis by the kidney and other tissuesNature, 1985
- The human LDL receptor: A cysteine-rich protein with multiple Alu sequences in its mRNACell, 1984
- The invariant chains of mouse class II antigens: biochemical properties and molecular relationshipEuropean Journal of Immunology, 1984
- Alternative RNA splicing in expression of the H–2KgeneNature, 1983
- Invariant proteins associated with guinea-pig Ia antigensMolecular Immunology, 1983
- Control of Thyroglobulin Synthesis and SecretionNew England Journal of Medicine, 1979
- Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segmentsNature, 1979
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975