Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer

Abstract

DNA-mediated gene transfer was used to identify cloned class I genes from the major histocompatibility complex of the BALB/c mouse. Three genes encoding the transplantation antigens H-2 Kd, Dd and Ld were identified as well as genes encoding the Qa-2,3 and 2 TL differentiation antigens. As many as 10 putative novel class I genes were detectred by the association of their gene products with .beta.2-microglobulin. Alloantiserum prepared to one of the novel antigens was used to demonstrate the expression of the previously undetected antigen on spleen cells of various inbred, congeneic and recombinant congeneic strains of mice.