Simultaneous Determination of Skeletal Muscle Fiber, Types I, IIA, and IIB by Histochemistry
- 1 March 1977
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of Neurology
- Vol. 34 (3), 171-173
- https://doi.org/10.1001/archneur.1977.00500150057011
Abstract
• We describe a simplified technique for the histochemical determination of three fiber types from a single section of skeletal muscle. Preincubation in a solution of formaldehyde, glycine, and calcium followed by routine myofibrillar adenosine triphosphatase (ATPase) incubation clearly differentiates type I, type IIA, and IIB fibers in human, rat, rabbit, and porcine muscle. In addition, glycineformaldehyde-calcium preincubation offers better preservation of cytoarchitecture and standardization of incubation time.This publication has 3 references indexed in Scilit:
- Definition of pathological changes seen in muscle biopsiesPublished by Elsevier ,2007
- Muscle Fiber Types: How Many and What Kind?Archives of Neurology, 1970
- FACTORS AFFECTING THE ACTIVITY OF ADENOSINE TRIPHOSPHATASE AND OTHER PHOSPHATASES AS MEASURED BY HISTOCHEMICAL TECHNIQUESJournal of Histochemistry & Cytochemistry, 1955