A simple method for incorporating aequorin into mammalian cells
- 1 August 1986
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 251 (2), C323-C326
- https://doi.org/10.1152/ajpcell.1986.251.2.c323
Abstract
A simple method for incorporating aequorin into mammalian cells to measure cytosolic ionized Ca2+ is described and compared with scrape loading and hypoosmotic treatment (HOST). The procedure consists of incubating the cells for 10 min and centrifuging them at 200 g for 30 s in the presence of aequorin. This method incorporates the same amount of photoprotein as scrape loading but 70% less than HOST. Cytosolic ionized Ca2+ has been measured in hepatocytes, kidney cells and tubules, macrophages, and cardiac myocytes loaded with aequorin by this new procedure.This publication has 5 references indexed in Scilit:
- Effects of low extracellular sodium on cytosolic ionized calcium. Na+-Ca2+ exchange as a major calcium influx pathway in kidney cells.Journal of Biological Chemistry, 1985
- Measurement of cytosolic calcium with aequorin in dispersed rat ventricular cellsJournal of Molecular and Cellular Cardiology, 1985
- Measurement of Intracellular Free Calcium in Monkey Kidney Cells with AequorinScience, 1982
- The permeability to calcium of pigeon erythrocyte ‘ghosts’ studied by using the calcium-activated luminescent protein, obelinBiochemical Journal, 1975