Dane Particle-Associated DNA Polymerase and e Antigen: Relation to Chronic Hepatitis among Carriers of Hepatitis B Surface Antigen

Abstract

Thirty-nine carriers of hepatitis B surface antigen (HB_s Ag) were studied with respect to e antigen and Dane particle-associated DNA polymerase activity and their relation to chronic hepatitis. Most of these individuals were followed for four or five years. A strong correlation between e antigen and DNA polymerase activity was found. Of the 22 e antigen-positive patients, 21 showed polymerase activity; none of the 13 e antigen-negative patients (one of whom had antibody to e antigen) had such activity. Three of four patients who became e antigennegative after being e antigen-positive showed loss of polymerase activity. An independent clinical evaluation showed a strong correlation between chronic hepatitis and positive reactions in the tests for e antigen and DNA polymerase. The results emphasize the possibility of differentiating between groups of chronic carriers of HB_s Ag by testing for e antigen and Dane particle-associated DNA polymerase activity. The differentiation may have important clinical implications.