EFFECTS OF CIS-DICHLORODIAMMINEPLATINUM(II) ON HUMAN-COLON CARCINOMA-CELLS INVITRO

Abstract

The lethal effects of cis-dichlorodiammineplatinum(II) [CDDP] were investigated on an established human colon carcinoma [LoVo] cell line. CDDP was one of the most efficient antineoplastic agents tested thus far on this human colon carcinoma cell line. Survival of exponentially growing cells exposed to increasing concentrations of the drug (both in medium or in Hanks'' balanced salt solution) was of the threshold exponential type (Dq [quasithreshold dose] = 1.2 .mu.g/ml, 1 h; D0 [mean lethal dose] = 3.5 .mu.g/ml, 1 h). Stationary-phase cells were extremely sensitive to the drug, and the survival curve demonstrated a simple exponential pattern (D0 = 3.9 .mu.g/ml, 1 h). Long-term exposure to low concentrations of CDDP induced a high degree of killing, with only 0.5% of the cells surviving after incubation for 24 h with 2 .mu.g/ml. Cells were unable to recover from potentially lethal or sublethal damages induced by the drug.