Iso-enzymes of acid ribonuclease in cotyledons of Pisum sativum L.
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 130 (2), 141-144
- https://doi.org/10.1007/bf00384411
Abstract
Acid ribonuclease from cotyledons of Pisum sativum is very stable, with a temperature optimum of 65° C. It has a molecular weight of 17,500 and there is evidence that a fragment, with a molecular weight of 3,100, retains enzyme activity. Acid ribonuclease from the cotyledons of five-day old seedlings may be fractionated into two iso-enzymes, I and II, by CM-cellulose chromatography. The increase in activity of iso-enzyme I is not inhibited by cycloheximide, whereas the increase in iso-enzyme II activity is strongly inhibited by cycloheximide. Cotyledons of 9-day old seedlings contain only iso-enzyme I, whilst cotyledons of 15-day old seedlings contain three iso-enzymes, I, IIa and III.Keywords
This publication has 9 references indexed in Scilit:
- Development of nuclease activity in cotyledons of Pisum sativum L.Planta, 1975
- Changes in activity of lysosomal ribonuclease following mechanical damage to leaves of Solanum tuberosum L.Planta, 1975
- The development of ribonuclease and acid phosphatase during germination of Pisum arvenseBiochemical Journal, 1974
- Acid ribonuclease from wheat germ: Purification, properties and specificityBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- De Novo Synthesis of Ribonuclease and β-1,3-Glucanase by Aleurone Cells of BarleyPlant Physiology, 1972
- Plant Nucleases. II. Properties of Corn Ribonucleases I and II and Corn Nuclease IPlant Physiology, 1968
- Increase in chromatin associated nuclease activity of excised barley leaves during senescence and its suppression by kinetinBiochemical and Biophysical Research Communications, 1968
- Purification and properties of a ribonuclease isolated from etiolated cucumber seedlingsArchives of Biochemistry and Biophysics, 1968
- Studies on ribonuclease in tobacco leaves I. Purification and propertiesBiochimica et Biophysica Acta, 1957