Specificity of pyoverdine-mediated iron uptake among fluorescent Pseudomonas strains
Open Access
- 30 September 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (10), 4865-4873
- https://doi.org/10.1128/jb.170.10.4865-4873.1988
Abstract
Pyoverdine-mediated iron transport was determined for seven fluorescent Pseudomonas strains belonging to different species. For all strains, cell or cell outer membrane and iron(III)-pyoverdine combinations were compared with their homologous counterparts in uptake, binding, and cross-feeding experiments. For four strains (Pseudomonas putida ATCC 12633, Pseudomonas fluorescens W, P. fluorescens ATCC 17400, and Pseudomonas tolaasii NCPPB 2192), the pyoverdine-mediated iron transport appeared to be strictly strain specific; pyoverdine-facilitated iron uptake by iron-starved cells and binding of ferripyoverdine to the purified outer membranes of such cells were efficient only in the case of the homologous systems. Cross-feeding assays, in liquid or solid cultures, resulted, however, especially for P. fluorescens ATCC 17400, in some discrepancies compared with uptake and binding assays, suggesting that growth experiments are the least likely to yield correct information on specificity of the pyoverdine-mediated iron transport. For the three other strains (P. fluorescens ATCC 13525, P. chlororaphis ATCC 9446, and P. aeruginosa ATCC 15692), cross-reactivity was demonstrated by the uptake, binding, and cross-feeding experiments. In an attempt to determine which parts of the iron transport system were responsible for the specificity, the differences in amino acid composition of the pyoverdines, together with the differences observed at the level of the iron-sensitive outer membrane protein pattern of the seven strains, are discussed.This publication has 63 references indexed in Scilit:
- Highly Efficient Uptake of a Rifamycin Derivative via the FhuA-TonB-dependent Uptake Route in Escherichia coliMicrobiology, 1987
- Siderophores: Their Biochemistry and Possible Role in the Biocontrol of Plant PathogensAnnual Review of Phytopathology, 1986
- Structure of pseudobactin 7SR1, a siderophore from a plant-deleterious PseudomonasBiochemistry, 1984
- Structure of ferric pseudobactin: a siderophore from a plant growth promoting PseudomonasBiochemistry, 1981
- Effect of iron on the protein composition of the outer membrane of fluorescent pseudomonadsFEMS Microbiology Letters, 1979
- Role of PyoverdinePf, the Iron-binding Fluorescent Pigment of Pseudomonas fluorescens, in Iron TransportJournal of General Microbiology, 1978
- The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical PropertiesJournal of General Microbiology, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- An Inverse Relationship of the Effects of Oxygen and Iron on the Production of Fluorescin and Cytochrome c by Pseudomonas fluorescensNature, 1963
- THE MINERAL REQUIREMENTS FOR FLUORESCIN PRODUCTIONCanadian Journal of Research, 1948