COLD ACETONE FIXATION FOR ENZYME LOCALIZATION IN FROZEN SECTIONS

Abstract

Frozen sections of unfixed tissues give better localization of enzyme activity after cold acetone treatment for 15 minutes. Acetone-fixed sections may be used for aminopeptidase, DPNH- and TPNH-tetrazolium reductases ("diaphorases"); succinate-tetrazolium reductase; DPN-linked lactic, malic, [beta]-hydroxybutyric, glutamic and [alpha]-glycerophosphate dehydrogenases; TPN-linked glucose-6-phosphate and isocitrate dehydrogenases; monoamine oxidase; ATPase, 5[image]-nucleotidase, and glucose-6-phosphatase activities.